Read about Hannibal Lector's menacing tic.
One of the creepiest elements of Anthony Hopkins's Oscar-winning portrayal of Hannibal Lector in The Silence of the Lambs is the fact that Lector never, ever blinks while talking on screen.
Hopkins picked up this physical trick to prepare for the role by watching videos of serial killers. He noticed that Charles Manson never blinked, and was compelled by the eerie quality of his gaze.
He also drew elements of Lector's voice and behavior from Truman Capote, Katherine Hepburn, and HAL 9000 of Stanley Kubrick's 2001: A Space Odyssey. Hopkins also invented the slurping noise Hannibal makes after his famous "fava beans and a nice chianti" line impromptu during shooting.